The experimental plan used could be modified by creating more constructs to give more data on the rheological properties and minimizing the impact of outliers.  CCK-8 kits would help to determine the number of cells for proliferation assay [5]. More tests could also be done to determine the swelling ratio, porosity, degradation, and cellular inoculation efficiency. Tests could also be done on the individual inks to determine the independent physical properties. Since MSCs are progenitors of chondrocytes growth factors, like TGF-β3, can be used to promote differentiation to chondrocytes. If budget and availability permit chondrocytes could be used directly and the differentiation step could be skipped. A longer culture of the constructs provides more information on the conditions of cell growth. A longer incubation time with chondrocytes would also give more information on the production of collagen and ECM components and the replacement of the scaffold. The constructs could then be measured to determine if the in vitro cartilage has similar properties to native cartilage. A computer model should be created so the material can be tested under different conditions. Using the custom material feature of Siemens NX, a finite element analysis can be done on the chosen material to determine the theoretical physical properties of the scaffold. The properties of each ink would need to be determined to be entered as a custom material.

Outside of changes to the established testing plan there are an endless number of changes that can be made. When printing doping a cell suspension to aggregate cells for culturing avoids pressure limitation in scaffold printing process [4]. This would allow the fidelity of scaffold printing to be enhanced without sacrificing cell viability during printing process. Additionally, the shape of the extrusion needle also affects the cell viability [20]. At low printing speed, a coaxial shape has a greater performance compared than other shapes. Various shapes could be trialed to determine if one could minimize the shear stress while printing. Additionally, rounded pores in scaffold are better than the typical lattice pattern in bioprinting [4] [21]. Different infill percentages could also be varied to determine which provides the optimal mechanical properties without compromising efficiency. Hydrogels have a promising future in the application of biomaterials and regenerative medicine. As more research in alginate and gelatin based bioinks and in cartilage regeneration is published more refinements and changes can be made.